By using polymers of different composition and assuming a triplet code one can deduce limited information about the composition of certain triplets.
More Quotes from Francis Crick:
It seems virtually certain that a single chain of RNA can act as messenger RNA, since poly U is a single chain without secondary structure.Francis Crick
Do codons overlap? In other words, as we read along the genetic message do we find a base which is a member of two or more codons? It now seems fairly certain that codons do not overlap.
Francis Crick
The balance of evidence both from the cell-free system and from the study of mutation, suggests that this does not occur at random, and that triplets coding the same amino acid may well be rather similar.
Francis Crick
Moreover the incorporation requires the same components needed for protein synthesis, and is inhibited by the same inhibitors. Thus the system is most unlikely to be a complete artefact and is very probably closely related to genuine protein synthesis.
Francis Crick
A comparison between the triplets tentatively deduced by these methods with the changes in amino acid sequence produced by mutation shows a fair measure of agreement.
Francis Crick
The meaning of this observation is unclear, but it raises the unfortunate possibility of ambiguous triplets; that is, triplets which may code more than one amino acid. However one would certainly expect such triplets to be in a minority.
Francis Crick
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